Magrose Protein G Magbeads for Protein Purification 30-150 μm, 10% (v/v)
1, Description:
This product is micron magnetic beads, skilled operation can complete the antibody adsorption process within 15 min, and complete the process of antibody purification within 30 min. The one-step solution can reduce the antibody absorption process to 10 minutes and purification process to 30 minutes, yielding antibody with >90% purity. It can be used for antibody fixation and other related studies. By referring the attached Table 1 users can select the categories of magnetic beads according to the source and subtype of the target antibody, and compare the affinity of Magrose Protein A and Magrose Protein G beads with different antibodies.
2, Product Information:
| Product Name |
Magrose Protein A
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Bead Size
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30~150 μm
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Beads Concentration
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10% (v/v)
|
|
Ligand
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Protein A
|
|
Medium
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Magrose
|
|
Antibody Bonding Quantity
|
25~30 mg Human IgG/mL Gel
|
|
Storage Temperature
|
2~8℃
|
| Binding/Washing Buffer |
PBST (pH 7.2~7.4): 137 mM NaCl,2.7 mM KCl, 10 mM
Na2HPO4, 2.0 mM KH2PO4, 0.1% Tween-20
|
|
Elution buffer
|
100 mM Gly, 0.1% Tween-20, pH 2.5
|
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Neutrilization buffer
|
1.0 M Tris-HCl, pH 9.0
|
|
Storage buffer
|
PBST (includes 0.05% NaN3)
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|
Shelf life
|
12 months
|
3, Feature:
1. Highly effective antibody binding capacity and ultra-low non-specific adsorption performance
2. Time saving, simple and mild
3. High product stability
4. Antibody elution system is closer to neutral
5. Very low protein A ligand off rate
6. Reusable
4, FAQ:
Q1: How to avoid possible aggregation of magnetic beads during storage or use?
A1: Beads should be stored at 2~8℃, and avoid for irreversible aggregation due to contamination or drying. Magnetic beads aggregation at low pH elution buffer is a normal phenomenon, and it does not affect the normal use of magnetic beads. In Binding/Washing buffer and Elution buffer adding with the final concentration of 0.1% (v/v) nonionic detergent (such as NP-40, Tween-20 or Triton X-100) can effectively prevent the beads
aggregation. The magnetic beads in low pH elution can be washed to neutral with Binding/Washing buffer and Elution buffer, and process with 2 min ultrasonic water bath. It can restore the magnetic beads to homogeneity, and the above treatments had no effect on the antibody binding efficiency with magnetic beads.
Q2: Magnetic beads appear agglomeration in the process of using?
A2: The agglomeration phenomenon is generally difficult to shake and break, which easily lead to uneven distribution. The reason is mainly because the magnetic beads are placed in the magnetic field for too long, the magnetic beads firmly binding together. The magnetic beads can be dispersed by 2 min ultrasonic water bath, but it should be noted that the antibody captured in the sample solution can lost from ultrasonic treatment, so it’s not recommended to use this method until elution.
Product Tags:
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150 μm Magnetic Beads Protein Purification 10% Volume Ratio 5 mL Images
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